A fluorimetric study of the hydroxylation of biphenyl in vitro by liver preparations of various species.
نویسندگان
چکیده
1. A study has been made of the enzymic hydroxylation of biphenyl by liver microsomal preparations from 11 species of animals, by using a fluorescence method for the micro-estimation of the hydroxylation products, 2- and 4-hydroxybiphenyl. 2. Livers from all species examined produced 4-hydroxybiphenyl, but only those from mice, hamsters, cats, coypus and frogs produced 2-hydroxybiphenyl as well. 3. Adult rat and rabbit livers produced only the 4-isomer, but livers from the young of these species also produced the 2-isomer. 4. The properties and requirements of the 4-hydroxylating enzyme of rabbit liver were studied. 5. The results are discussed and it is suggested that the 2- and 4-hydroxylating enzymes are different.
منابع مشابه
High-pressure liquid chromatographic and other assays for biphenyl hydroxylation compared [proceedings].
McPherson et al. (1974) have suggested that the stimulation in uitro of liver microsomal biphenyl 2-hydroxylation activity is a characteristic and potentially diagnostic property of chemical carcinogens. Many carcinogens, e.g. benzo[a]pyrene, are metabolized by microsomal preparations to compounds that show a very strong fluorescence in the same spectral region as 2-hydroxybiphenyl. A similarit...
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عنوان ژورنال:
- The Biochemical journal
دوره 96 3 شماره
صفحات -
تاریخ انتشار 1965